Roy - do you understand about depth of field ? That's the fall-off in sharpness from the point of focus, to points further away and closer to the camera, than where you've focused.
Depth of field varies by aperture (the f-stop) and the distance from the camera of the point focused upon.
If you focus on something close up, all things being equal, the depth of field will be smaller than if you focus on something far away.
If you shoot at a very open aperture (a small f number) the depth of field will be smaller than if you shoot at a larger aperture.
There's a flat plane that's in focus, parallel to the camera, at the point where the camera focuses. So if you focus on someone's eyeball and they are facing you, their ear will be out of focus at a wide open aperture.
Same way if you focus on the front of a building - the back of the building is further away and will be softer as a result. Where you crop relative to that plane in space will have a big difference on how sharp or soft the result is.
As an extreme example. The 100mm is one of the very sharpest lenses I own. Here it is shooting at f2.8. You can see that where you would pick a crop out of that shot would tell a very different story about how sharp a lens it is. You have a similar, but perhaps less extreme situation above.
(click for a larger version)
Then also, the handholding, low light, camera shake etc all make an image much softer than it could be on a tripod. If you need to prove that to yourself, get a laser pointer and point it at a wall 6 feet away from you. Stop the point from moving. Now put the pointer on a table and stop it moving. The tripod is like that - even at high, fast shutter speeds.